Mouse PDI (Protein Disulfide Isomerase) ELISA Kit (MOES01408)

This colorimetric immunoassay from Assay Genie provides quantitative measurement of mouse Protein Disulfide Isomerase (PDI), the 57 kDa ER-resident thiol isomerase encoded by the P4HB gene. PDI catalyzes the formation, reduction, and rearrangement of disulfide bonds during oxidative protein folding and has attracted attention as a biomarker of ER stress, neurodegenerative disease progression, tumor invasion, and platelet-driven thrombosis.

The kit is supplied as a complete 96 assays pack containing pre-coated microplate wells, biotinylated detection antibody, recombinant mouse PDI standards, streptavidin-HRP conjugate, wash buffer, substrate solution, and stop reagent; absorbance is read on a standard plate reader. Workflows supported include quantification of circulating PDI levels in murine serum, analysis of plasma-derived samples, and measurement of PDI expression in tissue homogenates from mouse models of glioblastoma, hepatocellular carcinoma, Parkinson's disease, or type 2 diabetes.

Researchers using this Mouse PDI ELISA Kit from Assay Genie can integrate the assay into studies of UPR signaling, ischemia-reperfusion injury, and thrombus formation, with the entire pack configured as a single-use kit calibrated to the supplied recombinant standards.