Mouse PDGFD (Platelet Derived Growth Factor D) ELISA Kit (MOES01370)

Assay Genie's Mouse PDGFD kit runs as a complete quantitative workflow in approximately 3-4 hours from sample loading through stop-solution readout, with each microtitre plate supplied as 12 removable 8-well strips so researchers can size experiments without wasting wells. The format pairs a pre-coated capture antibody with a biotinylated detection antibody and streptavidin-HRP conjugate, generating signal via TMB substrate that is quantified after an acid stop step. Validated serum recovery typically falls within 85-100%, and intra- and inter-assay CV values remain under 10% across low, mid, and high-concentration control samples. The capture reagent specifically recognises the native mouse PDGFD homodimer with negligible cross-reactivity to related PDGF-A or PDGF-B isoforms, an important consideration when attributing fibroblast, pericyte, and smooth muscle cell activation to PDGFD signalling in cardiovascular, renal, and tumour-microenvironment studies. Reconstituted standards should be aliquoted to limit repeated freeze-thaw cycles; the sealed microplate pouch and lyophilised detection components tolerate brief ambient transit, and long-term reagent integrity is preserved under refrigeration with the plate shielded from light.