Mouse PDGFC (Platelet Derived Growth Factor C) ELISA Kit (MOES01369)

The Assay Genie Mouse PDGFC ELISA Kit quantifies Platelet Derived Growth Factor C, a secreted growth factor that activates PDGF receptors and drives angiogenesis, fibroblast proliferation, and tumor-stroma crosstalk. Researchers studying vascular remodeling, wound healing kinetics, or oncogenic signaling pathways use this kit to track circulating and tissue-level PDGFC concentrations in murine disease models.

The microplate is pre-coated with a capture antibody specific to mouse PDGFC, and the assay relies on a biotin-streptavidin-HRP detection cascade with TMB substrate. The protocol uses a 50 µL sample per well, with dilution recommended for high-concentration specimens.

Each kit contains a recombinant PDGFC standard for generating the calibration curve, biotinylated detection antibody, streptavidin-HRP conjugate, wash buffer concentrate, and stop solution. Reconstituted standards and detection antibody should be stored frozen at -20°C in single-use aliquots to preserve activity. Total assay time is approximately 3.5 hours from sample addition to final absorbance measurement.

For research use only.