Mouse PDE5A/cGMP-specific 3',5'-cyclic phosphodiesterase ELISA Kit

Designed for quantitative measurement of PDE5A in mouse samples, this Sandwich ELISA from Assay Genie provides a high-sensitivity tool for studying the cGMP signaling pathway. PDE5A is an enzyme that regulates cGMP levels, which are essential for smooth muscle relaxation and blood vessel dilation. Dysregulation of PDE5A has been implicated in diseases such as pulmonary hypertension and erectile dysfunction, making the kit a valuable resource for research into these pathologies and related therapeutic strategies.

The assay uses a double-antibody sandwich format with colorimetric detection. Compatible sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Sensitivity is 46.875 pg/mL with a working range of 78.125-5000 pg/mL. The kit should be stored at 2-8°C for 12 months.

The protocol involves equilibrating reagents and TMB substrate, followed by primary incubation at 37°C for 90 minutes, biotin-labeled detection antibody binding for 60 minutes, HRP-streptavidin binding for 30 minutes, and TMB color development in the dark for 10-20 minutes. Absorbance is read at 450 nm immediately after adding the stop solution.