Mouse PCNA (Proliferating Cell Nuclear Antigen) ELISA Kit (MOES01394)

The Assay Genie Mouse PCNA ELISA Kit provides quantitative measurement of Proliferating Cell Nuclear Antigen, a 36 kDa auxiliary factor of DNA polymerase delta that is upregulated during S-phase and serves as a widely adopted index of cellular proliferation in studies of tumor growth, hepatic regeneration, and stem cell expansion.

Samples of serum, plasma, and other biological fluids are added to the pre-coated microplate after standard preparation, with a typical sample volume of 50 uL per well. The sandwich ELISA format with colorimetric detection is intended for research use only, and kit reagents should be refrigerated at 2 to 8 degrees C until first use to preserve activity through the stated shelf life.

The mouse-specific antibody pair avoids the cross-reactivity concerns associated with some pan-species antibodies used in immunohistochemistry and western blot workflows, supporting accurate measurement of proliferation indices in murine cancer, toxicology, and tissue regeneration models.