Mouse PBEF/Visfatin ELISA Kit

From Assay Genie, this double-antibody sandwich ELISA kit enables the precise measurement of Visfatin — also known as PBEF (pre-B cell colony-enhancing factor) — in mouse samples, including serum, plasma, cell culture supernatants, cell or tissue lysates and other liquid sample types. The assay uses a colorimetric detection method and is intended for laboratory research applications in molecular biology and biomedical sciences.

Visfatin is described as a key protein involved in metabolic function and inflammation regulation, and is linked to conditions such as obesity, diabetes and cardiovascular disease, making it a valuable biomarker for research into these areas. The kit reports a sensitivity of 0.375 ng/mL, a measuring range of 0.625-40 ng/mL and storage at 2-8°C for 12 months. The associated UniProt entry is Q99KQ4, and reported synonyms include VF, NAMPT, NAmPRTase, NMPRTase and PBEF1.

Reported linearity for serum (n=5) at 1:2, 1:4 and 1:8 dilutions is 86-94%, 80-94% and 89-104% respectively, for EDTA plasma (n=5) 93-105%, 86-102% and 86-103%, and for heparin plasma (n=5) 93-100%, 83-100% and 81-96%. Recovery is reported as 85-104% (average 94%) for serum, 86-105% (average 93%) for EDTA plasma and 88-99% (average 96%) for heparin plasma.