Mouse Pax (Paxillin) ELISA Kit (MOES01341)

Quantify endogenous paxillin in murine specimens with this solid-phase immunoassay from Assay Genie. Paxillin is a 68 kDa focal adhesion phosphoprotein that anchors integrin-based adhesion complexes to the actin cytoskeleton, recruits FAK and Src family kinases, and is widely studied as a marker of cytoskeletal remodeling in tumor metastasis, angiogenesis, and cell migration.

The kit is configured as a microplate with removable strips for partial runs, supplied with pre-coated capture wells, lyophilized standard, biotinylated detection reagent, and streptavidin-HRP conjugate in individual vials. Reconstituted standards and detection antibody should be aliquoted and stored frozen to avoid repeated freeze-thaw cycles, while the sealed microplate pouch remains stable refrigerated until the printed expiration.

Researchers apply the assay to investigate integrin signaling dynamics, EMT progression in carcinoma models, focal adhesion turnover during wound healing, and cytoskeletal changes in knockout or transgenic mice, with incubation steps carried out at room temperature.