Mouse PAR-1/Proteinase-activated receptor 1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit enables quantitative measurement of Proteinase-Activated Receptor 1 (PAR-1) in mouse samples using a double antibody format with colorimetric detection. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay reports a sensitivity of 0.188 ng/mL and a working range of 0.313-20 ng/mL, and kits are stored at 2-8°C for 12 months. The product is positioned for cardiovascular research applications.

PAR-1 is described as a critical receptor mediating cellular responses to proteases, with roles in inflammation, thrombosis and cancer progression, so accurate quantification supports studies of disease development and potential therapeutic strategies. Linearity was assessed at 1:2, 1:4 and 1:8 dilutions (n=5 each), with values of 87-104% in serum, 83-101% in EDTA plasma and 81-99% in heparin plasma. Recovery testing across the same matrices gave averages of 99% for serum (range 87-105%), 95% for EDTA plasma (range 89-99%) and 95% for heparin plasma (range 86-104%).