Mouse pAMPK/Phosphorylated Adenosine Monophosphate ActivatedKinase ELISA Kit

A colorimetric Sandwich ELISA kit from Assay Genie designed for the quantitative detection of phosphorylated adenosine monophosphate-activated kinase (pAMPK) in mouse samples. Built on a double-antibody sandwich format with TMB substrate development and absorbance read at 450 nm, it is positioned for research on cellular energy balance, metabolism, and homeostasis, with relevance to metabolic disorders, diabetes, obesity, and cancer.

The assay is validated for serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. Reported sensitivity is 0.188 ng/mL and assay range is 0.313–20 ng/mL. The kit should be stored at 2–8°C for up to 12 months. Linearity across 1:2, 1:4, and 1:8 dilutions (n = 5) was 87–102%, 87–98%, and 87–100% for serum; 85–103%, 86–95%, and 84–99% for EDTA plasma; and 85–100%, 87–100%, and 86–99% for heparin plasma. Spike-and-recovery (n = 5) ranged from 85–105% with an average of 96% in serum, 89–98% averaging 93% in EDTA plasma, and 85–105% averaging 92% in heparin plasma.

The workflow includes a 90-minute primary incubation at 37°C, a 60-minute biotinylated detection antibody step, a 30-minute HRP-Streptavidin (SABC) incubation, and TMB color development in the dark for 10–20 minutes before stop solution is added and absorbance is read at 450 nm. The product is cited in Elmahboub et al., Molecules 2024 (PubMed ID: 38611893).