Mouse Palate/Lung And Nasal Epithelium Associated Protein (PLUNC) ELISA Kit

Researchers investigating innate mucosal immunity in murine models can quantify PLUNC protein levels using this Assay Genie ELISA Kit, a microplate-based immunoassay intended for research use only. PLUNC, also referred to as SPLUNC1 or BPIFA1, is a secreted defense protein expressed throughout the upper respiratory tract, including nasal epithelium, trachea, and palate, where it contributes to antimicrobial activity and regulation of airway surface liquid composition.

The kit supports 96 assay determinations and is suited to measuring PLUNC across mouse-derived matrices, supporting investigations into respiratory host defense, sinonasal inflammation, cystic fibrosis models, and epithelial barrier biology. The procedure produces an absorbance-based readout compatible with standard laboratory plate readers, and components should be stored at 4°C upon arrival to preserve reagent stability through the shelf life of the kit.

Typical applications include profiling PLUNC in nasal and lung tissue homogenates and in liquid specimens derived from mouse models of infection, allergic airway disease, or environmental exposure. The enclosed protocol describes standard workflows including sample incubation, washing, and substrate addition for reproducible in vitro quantification, with each microplate runnable in parallel to process multiple study samples efficiently.