Mouse P-Selectin Glycoprotein Ligand 1 (PSGL1) ELISA Kit

PSGL-1, the mucin-like glycoprotein displayed on leukocytes that engages P-selectin during the earliest adhesive contacts with vascular endothelium, is quantified in murine sample matrices by the Assay Genie Mouse PSGL-1 ELISA Kit, an in vitro colorimetric immunoassay intended for use with mouse serum samples, plasma samples, and tissue homogenate preparations. Reagents are configured for plate-based batch processing and include a biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and calibrated mouse PSGL-1 standards for construction of the standard curve on each run. Researchers working in inflammation, vascular biology, and cardiovascular disease apply this kit to follow disease-associated changes in circulating or shed PSGL-1 levels in mouse models of atherosclerosis, sepsis, sickle cell vaso-occlusion, and ischemia-reperfusion injury, where ADAM-family metalloproteinase shedding provides a measurable readout of endothelial activation. Refrigerated storage of sealed components and avoidance of repeated freeze-thaw cycles of reconstituted standards help preserve reagent performance across multiple experimental runs. The kit is supplied for research use only and supports standardized quantitative comparison across treatment cohorts and genetic backgrounds in murine studies of leukocyte trafficking and selectin-mediated cell adhesion.