Mouse Ovalbumin Specific IgG/OVA sIgG ELISA Kit

An immunoassay from Assay Genie designed for the in vitro quantitative determination of Ovalbumin-specific IgG (OVA sIgG) concentrations in mouse serum, plasma, and cell culture supernatants. The kit uses an Indirect ELISA format with HRP detection and supports immunological research applications including vaccine development and antibody response monitoring.

Sensitivity is 1.875 ng/ml with an assay range of 3.125-200 ng/ml. Intra-assay precision is CV <8% and inter-assay precision is CV <10%. Recovery (spiked matrices, n=5) was 94-103% for serum (average 99%), 86-104% for EDTA plasma (average 93%), and 88-102% for UFH plasma (average 94%). Linearity (percentage of calculated versus expected concentration) at 1:2, 1:4, and 1:8 dilutions was 86-104%, 91-105%, and 85-102% for serum; 83-101%, 83-92%, and 85-95% for EDTA plasma; and 82-98%, 80-98%, and 80-97% for UFH plasma.

Components include a dismountable ELISA microplate, lyophilized standard, sample/standard dilution buffer, HRP-labeled antibody concentrate, antibody dilution buffer, TMB substrate, stop solution, wash buffer, and plate sealer. Storage is 4°C for 6 months. For research use only.