Mouse Osteocalcin ELISA Kit

From Assay Genie, this sandwich ELISA kit with a double-antibody design is intended for the quantitative measurement of osteocalcin in mouse samples using a colorimetric detection method. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The kit is supplied as a solid-phase assay for research on bone metabolism, including the regulation of bone formation and mineralization, and on conditions such as osteoporosis, diabetes, and metabolic disorders in mouse models.

Manufacturer-stated performance characteristics include a sensitivity of 0.234 ng/mL and an assay range of 0.391-25 ng/mL. Reported linearity across serum, EDTA plasma and heparin plasma at 1:2, 1:4 and 1:8 dilutions spans 85-104%, while recovery averages 94% for serum, 97% for EDTA plasma, and 92% for heparin plasma (n = 5 per sample type). The protocol involves primary incubation at 37°C for 90 minutes, biotin-labelled detection antibody binding for 60 minutes, HRP-streptavidin (SABC) binding for 30 minutes, TMB substrate development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution addition.

Reactivity is limited to mouse samples, and the kit is specified for storage at 2-8°C for up to 12 months.