Mouse Orphanin FQ/Nociceptin ELISA Kit

From Assay Genie, this competitive ELISA kit is designed for the accurate detection of Orphanin FQ/Nociceptin levels in mouse samples, including serum, plasma, cell culture supernatants, cell or tissue lysate, and other liquid samples. The assay delivers high sensitivity and specificity for reliable and reproducible results across a range of research applications.

Orphanin FQ/Nociceptin is a neuropeptide involved in pain modulation and stress responses, making it a valuable biomarker for studying pain pathways, addiction, and mental health disorders. The colorimetric assay uses antigen-coated wells and has a sensitivity of 4.688 pg/mL with a measuring range of 7.813-500 pg/mL.

Storage is at 2-8°C in sealed conditions; cryopreservation is not recommended. Reported recovery averages are 95% for serum, 93% for EDTA plasma, and 93% for heparin plasma. Linearity across 1:2, 1:4 and 1:8 dilutions spans 87-105% (serum), 85-100% (EDTA plasma) and 88-105% (heparin plasma) at the 1:2 dilution. Absorbance is read at 450 nm following TMB substrate colour development.