Mouse Ornithine carbamoyltransferase, mitochondrial (Otc) ELISA Kit

Designed for the quantitative measurement of mouse Ornithine carbamoyltransferase (OTC) in biological samples, this Sandwich ELISA Kit from Assay Genie delivers accurate and reproducible results. The assay is reactive with mouse samples and has been validated for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, with specificity for natural and recombinant mouse Ornithine carbamoyltransferase, mitochondrial.

Performance characteristics include a detection range of 0.312-20ng/ml, a sensitivity of 0.162ng/mL, an intra-assay CV of 4.1% and an inter-assay CV of 6.5%. Linearity has been demonstrated for serum, EDTA plasma and heparin plasma across dilutions from 1:2 to 1:16. Reported recovery is 80% (range 80-85%) for serum and 81% (range 80-87%) for plasma.

OTC is a mitochondrial enzyme of the urea cycle, where it catalyses the conversion of L-ornithine to L-citrulline and supports the detoxification of ammonia. Dysregulation of OTC activity has been associated with various metabolic disorders. The target is a homotrimer localised to the mitochondrial matrix (UniProt P11725; molecular weight 39,765 Da). For research use only.