Mouse Ornithine Carbamoyl Transferase/OCT ELISA Kit

This Sandwich ELISA Kit from Assay Genie is designed for the quantitative detection of mouse Ornithine Carbamoyl Transferase (OCT), a urea-cycle enzyme that plays a central role in nitrogen metabolism. The assay uses a colorimetric, double-antibody sandwich format on a pre-coated microplate, with absorbance read at 450 nm. Reactivity is specific to mouse samples.

Validated matrices include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. According to the manufacturer datasheet, the kit provides a sensitivity of 0.938 ng/mL and a working range of 1.563–100 ng/mL. Reported linearity for serum, EDTA plasma, and heparin plasma spans 80–104% across serial dilutions, and spike-and-recovery values for the same matrices fall between 85–105%. The kit should be stored at 2–8°C for up to 12 months.

Assay Genie positions this kit for research into metabolic disorders, noting that dysregulation of OCT has been linked to conditions such as hyperammonemia and liver disease, supporting its use as a biomarker for studying the urea cycle and related physiological processes.