Mouse OPG (Osteoprotegerin) ELISA Kit (MOES00685)

A Sandwich ELISA kit from Assay Genie for the quantitative measurement of Osteoprotegerin (OPG) in mouse serum, plasma, and cell culture supernatants. Osteoprotegerin is described as a key regulator of bone metabolism, acting as a decoy receptor for RANKL and inhibiting osteoclast differentiation and bone resorption. The kit uses a colorimetric detection method with an assay time of 3 h 30 min.

Performance characteristics include a sensitivity of 37.5 pg/mL and a detection range of 62.5-4000 pg/mL, with reported recovery of 80%-120%. The required sample volume is 100 μL. Specificity testing showed no significant cross-reactivity or interference between Mouse OPG and analogues. Kit components are stored at 2-8°C, with selected reagents stored at -20°C and protected from light.

The assay follows the Sandwich-ELISA principle using a biotinylated detection antibody and Avidin-Horseradish Peroxidase (HRP) conjugate, with absorbance measured at 450 nm ± 2 nm. Synonyms for the target include TNFRSF11B, OCIF, and TR1 (UniProt ID: O08712). Listed research areas include Cancer, Cardiovascular, Metabolism, Immunology, and Signal Transduction.