Mouse OBTF2 (Octamer Binding Transcription Factor 2) ELISA Kit (MOES01322)

Quantify Octamer Binding Transcription Factor 2 (OBTF2), a POU-homeobox transcription factor implicated in B-cell maturation and immunoglobulin gene regulation, in mouse specimens with this quantitative immunoassay from Assay Genie.

The kit is configured for 96 assays per box and ships with pre-coated microwell strips, biotinylated detection antibody, recombinant standards, streptavidin-HRP conjugate, wash buffer, chromogenic substrate solution, and stop reagent.

Standards and diluted samples are added to the plate, after which a chromogenic readout is captured on a standard microplate reader; absorbance values are interpolated against the generated standard curve to determine OBTF2 concentration. Intended for research use only, the assay works with mouse serum samples, plasma samples, cell lysates, and tissue homogenates, supporting studies of lymphocyte transcription factor expression, knockout phenotype validation, and time-course experiments in murine models.

Unopened components should be stored at 4 °C, with the standard kept frozen at -20 °C in single-use aliquots to limit freeze-thaw loss. Each lot is validated by Assay Genie for linearity, intra-assay precision, and spike-and-recovery performance in mouse matrices.