Mouse OBTF1 (Octamer Binding Transcription Factor 1) ELISA Kit (MOES01321)

This quantitative immunoassay from Assay Genie measures mouse OBTF1 (Octamer Binding Transcription Factor 1), a ubiquitously expressed POU-domain transcription factor also known as Oct-1 or NF-A1. Researchers use it to quantify Oct-1 protein abundance in mouse-derived liquid specimens, supporting studies in immunology, B-cell biology, and transcriptional regulation.

OBTF1 binds the canonical octamer motif ATGCAAAT and drives expression of housekeeping genes, histone H2B, and immunoglobulin heavy and light chain promoters. Investigators commonly apply this ELISA workflow to profile Oct-1 dynamics in lymphocyte development, viral host-factor research (including herpes simplex virus replication), and cellular stress or metabolic studies, where altered Oct-1 activity has been linked to immune cell maturation and viral gene regulation.

The 96-assay configuration provides pre-coated microplate wells, matched antibody pairs, calibrated reference standards, substrate solution, and complete wash and dilution buffers. Reagents should be refrigerated at 4 degrees C upon receipt to preserve antibody binding activity, and the kit is intended for research use only, supporting reproducible quantitation of transcription factor levels across experimental replicates.