Mouse O3far1/Omega-3 fatty acid receptor 1 ELISA Kit

From Assay Genie, this Sandwich ELISA kit uses a double-antibody format for the quantitative measurement of mouse O3far1 (Omega-3 fatty acid receptor 1), with colorimetric detection and absorbance read at 450 nm. The kit is validated for mouse samples including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, and is intended for research applications in developmental biology.

Reported performance figures include a sensitivity of 0.469 ng/mL and a measuring range of 0.781-50 ng/mL. Recovery spans 87-99% in serum (average 92%), 89-103% in EDTA plasma (average 94%), and 85-98% in heparin plasma (average 92%). Linearity across 1:2, 1:4 and 1:8 dilutions runs 85-101% for serum, 88-105% for EDTA plasma and 91-102% for heparin plasma. Reagents are stored at 2-8°C for 12 months. The assay workflow involves plate and reagent equilibration, a 37°C primary incubation for 90 minutes, biotin-labelled detection antibody incubation for 60 minutes, HRP-Streptavidin binding for 30 minutes, and TMB development in the dark for 10–20 minutes before stop and reading.

Provided components include a pre-coated dismountable ELISA microplate, lyophilized standard, biotin-labelled antibody concentrate, HRP-Streptavidin conjugate (SABC), TMB substrate, sample, antibody and SABC dilution buffers, stop solution, wash buffer, plate sealer and a technical manual. O3FAR1 functions as a receptor for omega-3 fatty acids involved in inflammation, lipid metabolism and immune response.