Mouse NUP133 (Nucleoporin 133kDa) ELISA Kit (MOES01314)

The NUP133 target is a core scaffold component of the nuclear pore complex, anchoring NUP107 and NUP160 within the Y-complex to mediate nucleocytoplasmic transport of proteins and mRNA. Researchers studying NPC biogenesis, mitotic checkpoint fidelity, and steroid-responsive transcriptional regulation rely on quantitative antigen measurement to track expression changes across tissues.

This microplate-based quantitative immunoassay from Assay Genie is configured for serum, supernatant, and tissue homogenate workflows. Each vial of reagents supports standard curve generation across the working range, with the assay incubation performed at room temperature to stabilize antibody binding. Reconstituted standard should be aliquoted and stored frozen to limit freeze-thaw loss of immunoreactivity, and the strip wells remain stable when refrigerated between runs.

Typical research applications include characterization of podocyte injury models, investigation of congenital nephrotic syndrome mechanisms involving NUP133 variants, and profiling of nuclear pore complex assembly defects in embryonic stem cell differentiation studies. The kit is supplied with reagent volume sufficient for a full microplate run, and is intended for research use only, not for diagnostic procedures.