Mouse Nuclear Respiratory Factor 1 (NRF1) ELISA Kit

This quantitative immunoassay from Assay Genie is purpose-built to quantify Nuclear Respiratory Factor 1 (NRF1), a transcription factor central to mitochondrial biogenesis and the expression of nuclear-encoded respiratory chain subunits, in mouse-derived samples. NRF1 orchestrates oxidative phosphorylation gene programs and cooperates with Tfam to maintain mitochondrial DNA copy number, which is why accurate protein-level quantitation matters in studies of metabolic disease, cardiac remodeling, and skeletal muscle adaptation.

Provided in a 96 assays format, the kit accepts tissue homogenates and cell lysates harvested from murine models, giving researchers flexibility to interrogate NRF1 abundance across tissues and cell preparations. Typical applications include profiling brown adipose tissue activation, tracking mitochondrial remodeling in heart and muscle, and pairing protein readouts with transcript-level measurements of downstream NRF1 target genes.

Reagents should be refrigerated, with multiple freeze-thaw cycles avoided to preserve antibody binding activity. Always protect assay components from light and equilibrate reagents to room temperature before running the plate.