Mouse Ntrk1/TrkA ELISA Kit

From Assay Genie, this Mouse NTRK1 (TRKA) ELISA Kit is a sandwich ELISA built on a double antibody format, designed for the precise quantitative measurement of Neurotrophic Receptor Tyrosine Kinase 1 (NTRK1/TRKA) in mouse samples. Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay reports a sensitivity of 46.875 pg/mL and a working range of 78.125-5000 pg/mL.

NTRK1, also known as TRKA, plays a central role in nerve growth and development, the regulation of pain sensitivity, and neuronal survival. Dysregulation of NTRK1 has been implicated in Alzheimer's disease, Parkinson's disease, and chronic pain conditions, making it a valuable biomarker for neurological research. The kit should be stored at 2-8°C for 12 months.

Linearity across dilutions (1:2, 1:4, 1:8) was demonstrated in serum (91-102%, 87-97%, 83-97%), EDTA plasma (85-102%, 83-100%, 81-93%), and heparin plasma (88-98%, 83-92%, 80-93%), each with n = 5. Spike-and-recovery testing yielded average recoveries of 97% in serum (range 94-102%), 92% in EDTA plasma (range 85-100%), and 94% in heparin plasma (range 88-100%).