Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) CLIA Kit (MOES00381)

From Assay Genie, this sandwich chemiluminescence immunoassay enables the in vitro quantitative determination of Mouse NOS2/iNOS (Nitric Oxide Synthase 2, Inducible) in serum, plasma, and other biological fluids. The kit applies the Sandwich-CLIA principle, using a microplate pre-coated with an antibody specific to Mouse NOS2/iNOS, a biotinylated detection antibody, and an Avidin-Horseradish Peroxidase (HRP) conjugate. Relative Light Unit (RLU) values are read on a chemiluminescence immunoassay analyzer and are positively correlated with analyte concentration.

Performance characteristics include a sensitivity of 37.50 pg/mL and a detection range of 62.50–4000 pg/mL, with an assay time of 4.5 hours and a sample volume of 100 μL. The kit is reported to recognize Mouse NOS2/iNOS with no significant cross-reactivity or interference from analogues. Listed synonyms for the target include NOS2A, HEP-NOS, and I-NOS.

Spike-and-recovery values are reported for serum (range 88–102%, average 95%), EDTA plasma (range 89–102%, average 96%), and cell culture media (range 90–104%, average 96%). Both intra-assay and inter-assay CV are stated to be below 15%, with intra-assay CV values of 7.29–9.05% and inter-assay CV values of 8.77–11.1% across the tested samples.