Mouse Nodal (Nodal) ELISA Kit

This Sandwich ELISA Kit from Assay Genie provides colorimetric detection and is intended for the quantitative measurement of Nodal levels in mouse serum, plasma, and cell culture supernatants. Nodal is a pivotal protein responsible for various developmental processes, including embryogenesis and stem cell differentiation. Its dysregulation has been associated with developmental defects and diseases such as cancer and infertility, highlighting its importance as a biomarker for studying these conditions and exploring therapeutic interventions.

The kit has a detection range of 31.2-2000pg/mL and a sensitivity of 15.64 pg/mL, with reported precision values of an Intra CV of 6.4% and an Inter CV of 7.1%. Linearity has been demonstrated for serum, EDTA plasma, and heparin plasma at serial dilutions of 1:2, 1:4, 1:8, and 1:16 (N=5), and average recovery values are 91% for serum and 93% for plasma.

Validated sample types include serum, plasma, tissue homogenates, cell culture supernates, and other biological fluids, with specificity for natural and recombinant mouse Nodal. The target is a disulfide-linked homodimer that is secreted and is essential for mesoderm formation and axial patterning during embryonic development. For research use only.