Mouse NLRP1 (NLR Family, Pyrin Domain Containing 1) ELISA Kit

Designed by Assay Genie for the accurate quantification of NLRP1 levels in mouse samples, this sandwich ELISA kit uses a double antibody format with colorimetric detection. The assay delivers a sensitivity of 4.688 pg/mL and a working range of 7.813-500 pg/mL, making it suitable for research into NLRP1, a member of the NOD-like receptor family with established roles in the regulation of inflammatory responses and immune signaling pathways.

The kit accepts serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples, offering flexibility for studies of inflammatory disorders, autoimmune diseases, and infectious diseases where NLRP1 dysregulation has been reported. Linearity figures show serum (n=5) ranging from 87-103% at 1:2, 88-104% at 1:4, and 85-96% at 1:8; EDTA plasma (n=5) from 90-96%, 92-99% and 84-99%; and heparin plasma (n=5) from 84-98%, 88-98% and 90-99% across the same dilutions.

Recovery data supplied by Assay Genie report averages of 98% for serum, 96% for EDTA plasma, and 94% for heparin plasma. The workflow involves primary incubation at 37°C, biotin-labeled detection antibody binding, HRP-streptavidin binding, TMB color development, and absorbance measurement at 450 nm. Reagents are stored at 2-8°C for 12 months.