Mouse NKB  (Neurokinin B) ELISA Kit (MOFI01373)

Built around matched antibody pairs for quantitative measurement of Neurokinin B, this Assay Genie immunoassay targets the tachykinin-family decapeptide encoded by the Tac2 gene in mice. NKB is central to KNDy neuron signaling, where it co-localizes with kisspeptin and dynorphin in hypothalamic arcuate nucleus neurons, driving pulsatile GnRH release and gating the onset of puberty. Researchers in reproductive neuroendocrinology apply the kit to investigate NKB concentrations in mouse blood-derived specimens, supporting studies of polycystic ovary syndrome models, pre-eclampsia, hypogonadotropic hypogonadism, and pubertal timing.

The 96 Assays format includes a pre-coated microplate, lyophilized recombinant NKB standards in vials, biotinylated detection reagent, streptavidin-HRP, wash and substrate buffers, and a stop reagent. Unopened components should be refrigerated upon receipt; once reconstituted, standards and detection antibody are commonly aliquoted and frozen to limit freeze-thaw cycles. The absorbance-based readout is captured with a standard microplate reader, and the assay is intended for in vitro research use only, suitable for knockout mouse phenotyping, pharmacological intervention studies, and neuropeptide pathway characterization.