Mouse Nitric Oxide Synthase 3, Endothelial (NOS3) ELISA Kit

The Assay Genie Mouse NOS3 ELISA Kit targets endothelial nitric oxide synthase, the enzyme that produces nitric oxide in vascular endothelial cells. Because eNOS expression and activity are closely tied to endothelial homeostasis, quantitative measurement of this protein supports research in vascular biology, cardiovascular pathophysiology, and inflammation-related signaling pathways. The immunoassay accommodates mouse-derived inputs such as tissue homogenates and cell lysates, with a recommended sample volume of 100 µL per well for standard loading, enabling studies in animal models of atherosclerosis, hypertension, diabetes, and ischemia-reperfusion injury. Reagents should be stored refrigerated and protected from light, with repeated freeze-thaw cycles avoided by aliquoting working stocks. The assay follows a standard in vitro research workflow on a microplate, with results read on a standard plate reader and interpreted against a calibrator curve. Intended for research use only.