Mouse Nidogen-1 (Nid1) ELISA Kit

A high-quality Sandwich ELISA from Assay Genie designed for the quantitative detection of Mouse Nidogen-1 (Nid1) levels. The kit is validated for use with mouse serum, plasma, and cell culture supernatants, and is also applicable to tissue homogenates and other biological fluids. It detects both natural and recombinant mouse Nidogen-1.

Nidogen-1, also known as Entactin, NID-1, or Ent, is a sulfated glycoprotein widely distributed in basement membranes and tightly associated with laminin, collagen IV, and perlecan. It has a probable role in cell-extracellular matrix interactions, including cell adhesion, migration, and tissue development. Dysregulation has been linked to cancer, fibrosis, and neurological disorders, making it a valuable research biomarker.

The assay offers a detection range of 0.156–10 ng/mL with a sensitivity of 0.082 ng/mL. Intra-assay precision is 6.9% and inter-assay precision is 8.3%. Linearity ranges from 86–119% across serum, EDTA plasma, and heparin plasma samples, with recovery of 92–106%. For research use only.