Mouse NFIL-3 ELISA Kit

A sandwich ELISA kit from Assay Genie for the detection of NFIL-3 in mouse samples using a colorimetric readout. The kit is reactive with mouse and is intended for cell biology research, in particular studies of immune regulation, circadian rhythms, and associated disease mechanisms including autoimmune disorders and cancer.

It accepts a broad range of liquid sample types, including serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. According to the manufacturer's specifications, the assay sensitivity is 0.188 ng/mL and the working range is 0.313-20 ng/mL. The kit is shipped and stored at 2-8°C and remains stable for 12 months under those conditions.

The workflow follows a standard sandwich ELISA format with a biotin-labeled detection antibody and HRP-streptavidin: coated-plate incubation at 37°C for 90 minutes, detection antibody incubation for 60 minutes, HRP-streptavidin binding for 30 minutes, TMB color development in the dark for 10-20 minutes, and absorbance measurement at 450 nm after stop solution. The target analyte, NFIL3 (UniProt O08750), is a transcription factor implicated in maintaining immune homeostasis.