Mouse Neurophysin-2 PharmaGenie ELISA Kit (SBRS0062)

The Mouse Neurophysin-2 ELISA Kit from Assay Genie quantifies the vasopressin-associated carrier protein (NPII), which is co-secreted alongside antidiuretic hormone from posterior pituitary axon terminals. This Competitive ELISA, validated specifically for mouse NPII with documented cross-reactivity to rat neurophysin-2, supports research into hypothalamic-neurohypophyseal function, water balance regulation, and murine models of diabetes insipidus and SIADH. The 96-assay format is calibrated for cell culture supernatant, plasma, and serum specimens, with a standard curve spanning 0.156–10 ng/mL; 50 µL of sample is added per well in duplicate. The kit ships with a pre-coated microplate, competitive peptide standard, biotinylated detection antibody, and colorimetric substrate. Researchers studying neuroendocrine peptide processing, Brattleboro rat phenotype comparisons, or osmoregulatory gene knockouts can integrate this assay with downstream western blot or immunohistochemistry workflows. Reconstituted reagents should be refrigerated and protected from light to maintain binding activity through repeated freeze-thaw cycles.