Mouse Neuregulin 4 (NRG4) ELISA Kit

This immunoassay from Assay Genie delivers quantitative measurement of mouse Neuregulin 4 (NRG4) for research into metabolic regulation, brown adipocyte signaling, hepatic lipid handling, and type 2 diabetes pathophysiology. NRG4 is an EGF-family ligand implicated in energy expenditure, fatty acid oxidation in the liver, and systemic glucose homeostasis, making this reagent relevant to studies of obesity, non-alcoholic fatty liver disease, and insulin resistance.

The assay uses a capture antibody pre-coated onto the plate, paired with a detection antibody that produces an absorbance-based signal proportional to NRG4 concentration. The standard curve is generated from calibrated recombinant mouse NRG4 and is intended for mouse serum samples, plasma aliquots, tissue homogenate, and related specimen types. The kit includes a pre-coated plate, recombinant standards in vials, detection antibody, sample and standard diluents in bottles, concentrated wash buffer, substrate, and stop solution—sufficient reagents for a full plate run.

Typical applications include biomarker quantification in metabolic disease models, evaluation of NRG4 expression in liver and adipose tissues, screening of compounds affecting NRG4 signaling, and characterization of transgenic or knockout mouse lines. The straightforward workflow suits both academic and industrial research laboratories, with results used to investigate NRG4 as a candidate biomarker for metabolic disorders.