Mouse Neprilysin PharmaGenie ELISA Kit (SBRS1487)

The neprilysin PharmaGenie immunoassay from Assay Genie provides researchers with a quantitative tool to measure mouse neprilysin (also known as CD10, neutral endopeptidase, or MME) in experimental samples. Neprilysin is a zinc-dependent metallopeptidase that cleaves amyloid-beta peptide, natriuretic peptides, bradykinin, substance P, and other vasoactive substrates, making it a target of interest in Alzheimer's disease research, cardiovascular pharmacology studies involving neprilysin inhibitors, and investigations of renal and vascular function.

The kit is configured for 96 assays and supports work with cell culture supernatants, plasma, and serum samples of mouse origin. Each plate ships with pre-coated wells, detection antibody, lyophilized standards, and the buffers needed to build a standard curve and quantify neprilysin levels across experimental conditions. Researchers can apply the assay to supernatant harvested from cultured neurons or glial cells, to mouse plasma collected in EDTA or heparin tubes, and to serum prepared by standard clotting protocols.

Typical applications include measuring neprilysin expression in transgenic mouse models of neurodegeneration, screening compounds that modulate metalloprotease activity, profiling kidney or brain tissue lysates, and comparing circulating neprilysin concentrations between treatment groups in cardiovascular studies. The kit is intended for research use only in laboratory investigation of metalloprotease biology and is not cleared for clinical diagnostics.