Mouse Nephrin (NPHN) ELISA Kit

The Mouse Nephrin (NPHN) ELISA Kit from Assay Genie is engineered to quantify nephrin (NPHS1), a transmembrane signaling protein that forms the structural backbone of the slit diaphragm between adjacent podocyte foot processes in the renal glomerulus. Researchers use this assay to investigate podocyte injury, glomerular filtration barrier dysfunction, and proteinuria in murine models of diabetic nephropathy, focal segmental glomerulosclerosis, and congenital nephrotic syndrome. The microplate format includes pre-coated wells, biotinylated detection antibody, streptavidin-HRP conjugate, TMB substrate, and assay buffer components. Intended for research use only, the kit supports quantitative analysis of podocyte damage markers in murine urine, kidney tissue homogenates, and primary podocyte lysates. Reagents should be refrigerated upon arrival and equilibrated to room temperature before initiating the protocol.