Mouse NCAD (Neural Cadherin) ELISA Kit (MOES00789)

The Assay Genie sandwich format delivers reproducible performance with intra-assay CV under 10% and inter-assay CV under 12%, supporting confident comparison across mouse cohorts. The capture and detection antibodies target the extracellular domain of CDH2 and show negligible cross-reactivity with E-cadherin or P-cadherin, an important consideration when cadherin isoforms are co-expressed in developmental or tumor tissue. Each microplate runs in approximately 3.5 hours, and the limit of detection sits near 0.05 ng/ml. Serum and EDTA-plasma samples generally require 1:2 to 1:4 dilution in assay buffer, while cell culture supernatant can often be loaded neat depending on cell line and confluence. Tissue homogenates from brain or tumor xenografts are typically clarified at 10,000 x g after lysis in PBS with protease inhibitors before loading. Reconstituted standards and biotinylated detection antibody remain stable through several freeze-thaw cycles when aliquoted and stored at -80 °C, though repeated cycling should be limited to preserve binding activity.