Mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (Ndufa6) ELISA Kit

Assay Genie offers a sandwich ELISA kit for the quantitative detection of mouse NADH dehydrogenase [ubiquinone] 1 alpha subcomplex subunit 6 (NDUFA6), an accessory subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed not to be involved in catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain, with ubiquinone as the immediate electron acceptor, and NDUFA6 localizes to the mitochondrial inner membrane as a peripheral membrane protein on the matrix side.

The kit uses a colorimetric detection method and is reactive with mouse samples. It is validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and detects natural and recombinant mouse NDUFA6. The reported detection range is 0.156-10ng/mL, with a sensitivity of 0.069ng/mL. Linearity has been evaluated in serum (N=5), EDTA plasma (N=5) and heparin plasma (N=5) across 1:2, 1:4, 1:8 and 1:16 dilutions, with serum ranging from 85-125%, EDTA plasma from 88-118%, and heparin plasma from 91-116%. Research use only.