Mouse NACHT, LRR and PYD domains-containing protein 3 (Nlrp3) ELISA Kit

Designed for research use only, this Sandwich ELISA Kit from Assay Genie enables quantitative detection of mouse NACHT, LRR and PYD domains-containing protein 3 (Nlrp3). The assay is reactive with mouse samples and validated for serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Specificity covers natural and recombinant mouse Nlrp3, also known by synonyms including Cryopyrin, Cold autoinflammatory syndrome 1 protein homolog, Mast cell maturation-associated-inducible protein 1, PYRIN-containing APAF1-like protein 1, Cias1, Mmig1, Nalp3, and Pypaf1.

Performance characteristics include a detection range of 0.156-10 ng/mL and a sensitivity of 0.058 ng/mL. Reported precision is an intra-assay CV of 4.9% and an inter-assay CV of 7.1%. Recovery averages 93% (range 87-99%) in serum and 95% (range 89-101%) in plasma. Linearity for serum samples ranges from 101-119%, for EDTA plasma from 92-116%, and for heparin plasma from 91-127%, across serial dilutions of 1:2 to 1:16.

Nlrp3 is a cytoplasmic protein that interacts with PYCARD/ASC as part of the NALP3 inflammasome complex. According to the source, it may function as an inducer of apoptosis and as an upstream activator of NF-kappa-B signaling, while inhibiting TNF-alpha induced activation and nuclear translocation of RELA/NF-KB p65. The inflammasome activates caspase-1 in response to triggers including bacterial or viral infection, leading to processing and release of IL1B and IL18. The research area is Immunology, and the associated UniProt accession is Q8R4B8.