Mouse N-acetylmuramoyl-L-alanine amidase (Pglyrp2) ELISA Kit

From Assay Genie, this Sandwich ELISA kit is designed for the quantitative measurement of mouse N-acetylmuramoyl-L-alanine amidase (PGLYRP2), also known as Peptidoglycan recognition protein 2. Reactivity is mouse, and the assay is for research use only, detecting natural and recombinant mouse PGLYRP2 in serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Detection is colorimetric, with absorbance read at 450 nm.

Manufacturer-reported performance figures include a detection range of 78–5000 pg/mL and a sensitivity of 39.3 pg/mL. Intra-assay CV is 5.3% and inter-assay CV is 7.8%. Mean recovery is 90% in serum (range 84–96%) and 92% in plasma (range 86–98%). Dilutional linearity (N=5) across the 1:2 to 1:16 dilution series spans 80–107% for serum, 85–114% for EDTA plasma, and 95–119% for heparin plasma.

PGLYRP2 is annotated as a Secreted/Membrane protein with UniProt accession Q8VCS0, NCBI Gene ID 57757 and a molecular weight of 49,063 Da. Synonyms listed for the target include Peptidoglycan recognition protein long, TagL, PGRP-L, Pglyrpl and Pgrpl. The protein is described as a scavenger that digests biologically active peptidoglycan into biologically inactive fragments, with no direct bacteriolytic activity.