Mouse Myosin-11 (Myh11) ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative measurement of mouse Myosin-11 (MYH11). The kit is intended for the measurement of MYH11 levels in mouse serum, plasma, and cell culture supernatants and is supplied for research use only. According to the manufacturer, the kit offers high sensitivity and specificity for precise and reproducible results across a range of research applications.

MYH11 is described as a key protein involved in smooth muscle contraction and is essential for proper muscle function. Dysregulation of MYH11 has been linked to conditions such as hypertension and cardiovascular diseases, highlighting its relevance as a potential therapeutic target. The assay covers a working range of 0.312-20 ng/mL.

Sample handling guidance is given for serum (clotting followed by centrifugation at 1,000 × g) and plasma (EDTA or heparin as anticoagulant, centrifugation at 4°C for 15 minutes at 1000 × g within 30 minutes of collection), with aliquoting and storage at -80°C recommended to avoid multiple freeze-thaw cycles. A microplate reader capable of measurement at 450 nm is required.