Mouse MyD88 (Myeloid Differentiation Factor 88) ELISA Kit (MOES01822)

Performance characteristics include an intra-assay CV of 6.9%, inter-assay CV of 8.6%, and mean recovery of approximately 99% across representative sample matrices, with no significant cross-reactivity reported for related TLR or IL-1 receptor adapter proteins. Each well requires a 100 ul sample volume, and the full workflow completes in roughly 3 hours once samples have equilibrated to room temperature. The Assay Genie format supports downstream readouts in studies of LPS-driven inflammation, MyD88-dependent NF-κB activation in macrophage and dendritic cell lysates, and in vivo sepsis models where tissue homogenate MyD88 levels are tracked over time. Reconstituted standards should be aliquoted and stored frozen at -20 °C to limit freeze-thaw cycles, while unopened strips and remaining kit components are refrigerated at 2-8 °C per the product manual; the kit is intended for research use only.