Mouse MUP20/Major urinary protein 20 ELISA Kit

From Assay Genie, this competitive, antigen-coated ELISA kit is designed for the quantitative measurement of Mouse MUP20 (Major Urinary Protein 20) in research samples. MUP20 is a scent-signalling protein found in mice that plays a crucial role in chemical communication and social behaviour, and is essential for various physiological and behavioural processes. The kit supports studies of mouse biology and population-level interactions.

Validated sample types include serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. The assay has a sensitivity of 0.188 ng/mL and a working range of 0.313–20 ng/mL, with detection performed using a biotin-labelled antibody (100X) and HRP-streptavidin conjugate with TMB substrate; absorbance is read at 450 nm. Recommended storage is 2–8°C in a sealed state, and reagents should not be cryopreserved.

Linearity data for serum (n=10) at 1:2, 1:4 and 1:8 dilutions spans 85–101%, 88–98% and 81–97% respectively; EDTA plasma (n=10) 86–98%, 82–100%, 82–99%; heparin plasma (n=10) 88–100%, 82–99%, 82–100%. Recovery averages are 96% for serum and EDTA plasma, and 94% for heparin plasma, with corresponding ranges of 86–104%, 86–105% and 88–99%.