Mouse Mucin 1 (MUC1) ELISA Kit

Mouse mucin research in cell biology and oncology laboratories is supported by this MUC1 ELISA Kit from Assay Genie, supplied as a 96 assays pack containing pre-coated plate wells, detection reagents, and a recombinant standard for building a calibration curve. Absorbance is read on a standard microplate reader, and the assay is configured to quantify Mucin 1 across cell lysates, tissue homogenates, and cell culture supernatants from mouse-derived samples.

Mucin 1 (MUC1) is a heavily glycosylated transmembrane protein that carries a variable-number tandem repeat domain in its extracellular region. Aberrant O-glycosylation, overexpression, and shedding of the MUC1 ectodomain are hallmarks of adenocarcinomas of the breast, pancreas, ovary, and stomach, so quantitative tracking of MUC1 levels in mouse tumor models is widely used to monitor disease progression, evaluate vaccine or antibody-based immunotherapies targeting the tandem repeat, and characterize epithelial barrier changes in respiratory and gastrointestinal inflammation studies.

Workflow notes for this assay include running samples and standards in duplicate on the supplied microplate, allowing the standard curve to bracket the expected MUC1 concentration range. Reconstituted standard and concentrated detection antibody should be kept refrigerated and used within the kit shelf life to preserve activity. Researchers pair MUC1 quantification with downstream applications such as western blot confirmation of isoforms, immunohistochemistry on matched tissue sections, and flow cytometry analysis of cell-surface MUC1 to validate findings across orthogonal methods. The kit is intended for research use only and is not validated for diagnostic procedures.