Mouse MUC5AC (Mucin-5 subtype AC) ELISA Kit (MOES01275)

Assay Genie supplies a colorimetric, solid-phase sandwich ELISA for the quantification of mouse MUC5AC (Mucin-5 subtype AC) in serum, plasma and other biological fluids, including cell culture supernatants. The listed sensitivity is 0.1 ng/mL, the detection range is 0.16-10 ng/mL and the sample volume is 100 μL. The assay time is 3 h 30 min, and the kit is stored at 2-8°C.

Reported performance includes recovery of 80%-120%, an average recovery of 94% in EDTA plasma (range 87-101%) and 102% in cell culture media (range 95-112%). Intra-assay CV values are 6.78%, 6.46% and 5.69%, while inter-assay CV values are 7.23%, 7.31% and 7.9%. The kit recognises mouse MUC5AC, with no significant cross-reactivity or interference observed between MUC5AC and analogues. Supplied components include a dismountable micro ELISA plate, reference standard and sample diluent, biotinylated detection antibody, HRP conjugate, detection antibody and HRP conjugate diluents, wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis.

MUC5AC is a mucin protein involved in mucin secretion, mucociliary clearance and protection of the respiratory tract, and abnormal expression is associated with respiratory diseases including asthma, chronic obstructive pulmonary disease and cystic fibrosis. The kit is intended for research in cancer, immunology and signal transduction.