Mouse MT (Melatonin) ELISA Kit (MOES01266)

Assay Genie offers a competitive, colorimetric ELISA for measuring MT (melatonin) in mouse samples. The kit is intended for mouse serum, plasma and other biological fluids, including tissue culture supernatants and lysate samples, with a listed assay time of "2 h 30 min". The listed research areas are metabolism and neuroscience, and MT is also given as N-Acetyl-5-Methoxytryptamine.

Performance specifications include a sensitivity of "4.69 pg/mL", a detection range of "7.81-500 pg/mL" and a stated recovery of "80%-120%"; the specified sample volume is "50 μL". Reported intra-assay CV values were "4.56%", "4.43%" and "5.43%", while inter-assay CV values were "8.87%", "8.14%" and "8.45%". In recovery testing, EDTA plasma gave a range of "91-105%" with an average recovery of "97%", and cell culture media gave "86-98%" with an average recovery of "91%".

The manufacturer states that the kit recognizes mouse MT, with no significant cross-reactivity or interference observed between mouse MT and analogues. Storage is specified at "2-8℃". Components include a dismountable micro ELISA plate, reference standard, biotinylated detection antibody, HRP conjugate, sample and antibody diluents, concentrated wash buffer, substrate reagent, stop solution, plate sealer, technical manual and certificate of analysis. Listed research applications include investigating sleep disorders, mood disorders and neurodegenerative diseases.