Mouse Monofunctional C1-tetrahydrofolate synthase, mitochondrial (Mthfd1l) ELISA Kit

Assay Genie supplies a Sandwich ELISA kit for the quantitative measurement of mouse Monofunctional C1-tetrahydrofolate synthase, mitochondrial (Mthfd1l) using a colorimetric detection method. The kit is reactive with mouse samples, including serum, plasma, tissue homogenates, cell culture supernates and other biological fluids, and shows specificity for natural and recombinant mouse Monofunctional C1-tetrahydrofolate synthase, mitochondrial, a mitochondrial homodimer.

According to the manufacturer, performance specifications include a detection range of 1.56-100ng/mL, a sensitivity of 0.796ng/mL, an intra-assay CV of 8.4% and an inter-assay CV of 10.1%. Linearity has been evaluated in serum, EDTA plasma and heparin plasma across dilutions of 1:2, 1:4, 1:8 and 1:16. Reported recovery averages 90% (range 84-96) for serum and 92% (range 86-98) for plasma. The kit is supplied for research use only.

The target protein is described in the supplied literature as a key enzyme in folate and one-carbon metabolism that may provide the missing metabolic reaction required to link mitochondria and cytoplasm in the mammalian model of one-carbon folate metabolism in embryonic and transformed cells, complementing the enzymatic activities of MTHFD2. Dysregulation of MTHFD1L is reported to be linked to various diseases, including cancer, cardiovascular disorders and neurological conditions.