Mouse monoclonal antibody isotype ELISA Kit (MOFI01463)

The Assay Genie isotype identification panel enables simultaneous screening for the major murine immunoglobulin classes and subclasses most commonly encountered during hybridoma development, including IgG1, IgG2a, IgG2b, IgG3, IgA, and IgM. Researchers commonly apply 100 uL of diluted supernatant per well when screening conditioned media from cloned hybridoma lines, and the qualitative read-out supports rapid decisions on which wells to scale up for production. The strip-well layout lets unused wells be returned to the foil pouch with desiccant, reducing waste when only a few candidates need to be checked at a time. Reagents are shipped refrigerated and should be equilibrated to room temperature before use; bring the conjugate and wash buffer to ambient conditions rather than running the assay cold. Results are intended for research use only, supporting antibody characterization workflows such as clonality checks and subclass confirmation prior to purification.