Mouse MMP12 ELISA Kit

A sandwich ELISA from Assay Genie designed for the quantitative detection of mouse matrix metalloproteinase 12 (MMP12) levels in serum, plasma, cell culture supernatant, cell or tissue lysate, and other liquid samples. MMP12 is described by the manufacturer as a key enzyme involved in tissue remodeling and inflammation, with research relevance to chronic obstructive pulmonary disease (COPD), emphysema, and atherosclerosis.

The kit is intended for mouse samples and is stored at 2-8°C for 12 months. Associated identifiers from the manufacturer include the UniProt entry P34960 and the synonyms MMP-12, HME, ME, MME, Macrophage Metalloelastase, and Macrophage Elastase.

Validation data supplied by the manufacturer include linearity and recovery across serum, EDTA plasma, and heparin plasma. Linearity spans 82-105% across the three sample matrices at 1:2, 1:4, and 1:8 dilutions, and recovery averages between 93% and 97% depending on sample matrix. The procedure uses a biotin-labeled detection antibody with HRP-Streptavidin conjugate and TMB substrate, with absorbance read at 450 nm after a stop step.