Mouse MMP-8 (Matrix Metalloproteinase 8) ELISA Kit (MOES01263)

From Assay Genie, this Mouse MMP-8 (Matrix Metalloproteinase 8) ELISA Kit is designed for the quantitative detection of MMP-8 levels in mouse serum, plasma, and cell culture supernatants. Matrix metalloproteinase-8 (MMP-8) is an enzyme involved in extracellular matrix remodeling, specifically in the degradation of collagen, and its dysregulation has been linked to inflammatory diseases, cancer metastasis, and tissue injury. The kit provides researchers with a tool to study MMP-8 dynamics in mouse models and explore its role across various biological processes and diseases.

The kit employs a Sandwich-ELISA colorimetric method with an assay time of 3 h 30 min. Performance characteristics include a sensitivity of 0.19 ng/mL and a detection range of 0.31–20 ng/mL, with a reported recovery of 80%–120%. The micro ELISA plate is pre-coated with an antibody specific to Mouse MMP-8; bound target is detected using a biotinylated detection antibody followed by an Avidin-Horseradish Peroxidase (HRP) conjugate. The substrate reaction is terminated with stop solution, and the optical density is measured at 450 nm ± 2 nm, with the value proportional to target concentration as determined against a standard curve.

Validated sample types include serum, plasma, and other biological fluids at a sample volume of 100 μL. The kit is specific for Mouse MMP-8 (UniProt ID: O70138) with no significant cross-reactivity or interference observed with analogues. It is intended for storage at 2–8°C and is suited to research areas including Cancer, Cell Biology, Cardiovascular, and Signal Transduction.