Mouse Mitogen-activated protein kinase kinase kinase 5 (Map3k5) ELISA Kit

A sandwich ELISA kit from Assay Genie designed for the quantitative measurement of mouse Mitogen-activated protein kinase kinase kinase 5 (Map3k5). The assay is reactive with mouse samples and recognises both natural and recombinant forms of the target. Synonyms for the protein detected by this kit include Apoptosis signal-regulating kinase 1 (ASK-1), MAPK/ERK kinase kinase 5, and MEK kinase 5.

Detection is colorimetric and the kit is suitable for use with serum, plasma, tissue homogenates, cell culture supernates and other biological fluids. Quoted performance characteristics include a detection range of 0.312-20ng/mL and a sensitivity of 0.156ng/mL. Map3k5/ASK1 is a serine/threonine kinase that acts as an essential component of the MAP kinase signal transduction pathway and plays a crucial role in apoptosis through mitochondria-dependent caspase activation. It mediates signal transduction in response to oxidative stress and receptor-mediated inflammatory signals such as tumour necrosis factor (TNF) and lipopolysaccharide (LPS), and acts upon MAP kinase kinases to activate both the JNK and p38 MAPK cascades. The associated UniProt entry is O35099, and the kit is indicated for research use only within the neurosciences research area.