Mouse MIP-3 alpha (Macrophage Inflammatory Protein 3 Alpha) ELISA Kit (MOES00634)

Built on a classic sandwich ELISA architecture, this Assay Genie kit pairs a pre-titrated capture antibody with a biotinylated detection antibody and a streptavidin-HRP conjugate, so the final signal is read at 450 nm on a standard microplate reader after addition of stop solution. The recombinant Mouse CCL20 standard supplied with the kit supports a typical standard curve spanning roughly 15 to 1000 pg/mL, which enables quantification of endogenous chemokine levels in serum, plasma, and tissue culture supernatants. Reported intra-assay precision is generally below 10% CV when the protocol is followed closely. Researchers working in mucosal immunology, inflammatory bowel disease, psoriasis, and respiratory inflammation favor this target because CCL20 is the only known ligand for CCR6 and drives recruitment of Th17 cells and dendritic cells to inflamed tissue. For best results, bring all reagents to room temperature before use, avoid repeated freeze-thaw cycles of the reconstituted standard, and run each sample in duplicate to minimize well-to-well variation. The kit is intended for research use only and is not validated for diagnostic or therapeutic applications in mouse models.